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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.7

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        This report has been generated by the nf-core/chipseq analysis pipeline. For information about how to interpret these results, please see the documentation.

        Report generated on 2020-01-16, 05:47 based on data in: /home/jeremie/work1/98/30d85ef5c3d700331244757d0d15d8


        General Statistics

        Showing 392/392 rows and 32/40 columns.
        Sample NameM Reads MappedM Reads MappedM Reads MappedFrag LengthNSCRSC% Dups% GCLengthM Seqs% Trimmed% Dups% GCLengthM SeqsError rateM Non-PrimaryM Reads Mapped% MappedM Total seqsError rateM Non-PrimaryM Reads Mapped% MappedM Total seqsError rateM Non-PrimaryM Reads Mapped% MappedM Total seqs% Aligned% Dups
        D0_1_7_R1
        37.0
        29.2
        0
        1.04
        0.90
        0.23%
        0.0
        37.0
        99.4%
        37.2
        0.20%
        0.0
        29.2
        100.0%
        29.2
        100%
        D0_1_7_R1_T1
        37.0
        11.0%
        45%
        50 bp
        37.2
        1.7%
        10.5%
        45%
        49 bp
        37.2
        0.23%
        0.0
        37.0
        99.4%
        37.2
        9.3%
        D0_3_351_R1
        52.7
        45.0
        170
        1.23
        1.12
        0.22%
        0.0
        52.7
        99.8%
        52.8
        0.21%
        0.0
        45.0
        100.0%
        45.0
        100%
        D0_3_351_R1_T1
        52.7
        6.6%
        48%
        50 bp
        52.8
        1.0%
        6.4%
        49%
        49 bp
        52.8
        0.22%
        0.0
        52.7
        99.8%
        52.8
        5.9%
        D0_4_352_R1
        46.2
        38.2
        175
        1.32
        1.13
        0.23%
        0.0
        46.2
        99.8%
        46.2
        0.21%
        0.0
        38.2
        100.0%
        38.2
        100%
        D0_4_352_R1_T1
        46.2
        9.1%
        49%
        50 bp
        46.3
        1.0%
        8.8%
        49%
        49 bp
        46.2
        0.23%
        0.0
        46.2
        99.8%
        46.2
        8.5%
        D9_1_154_R1
        42.1
        18.6
        150
        1.31
        1.35
        0.22%
        0.0
        42.1
        98.2%
        42.9
        0.18%
        0.0
        18.6
        100.0%
        18.6
        100%
        D9_1_154_R1_T1
        42.1
        48.2%
        45%
        50 bp
        42.9
        1.3%
        46.1%
        45%
        49 bp
        42.9
        0.22%
        0.0
        42.1
        98.2%
        42.9
        46.3%
        D9_1_155_R1
        29.8
        21.5
        200
        1.21
        1.74
        0.23%
        0.0
        29.8
        99.4%
        30.0
        0.18%
        0.0
        21.5
        100.0%
        21.5
        100%
        D9_1_155_R1_T1
        29.8
        18.3%
        42%
        50 bp
        30.0
        1.2%
        17.4%
        42%
        49 bp
        30.0
        0.23%
        0.0
        29.8
        99.4%
        30.0
        15.8%
        D9_1_156_R1
        13.3
        9.9
        150
        1.03
        0.60
        0.25%
        0.0
        13.3
        99.0%
        13.5
        0.19%
        0.0
        9.9
        100.0%
        9.9
        100%
        D9_1_156_R1_T1
        13.3
        13.7%
        43%
        50 bp
        13.5
        1.2%
        13.1%
        44%
        49 bp
        13.5
        0.25%
        0.0
        13.3
        99.0%
        13.5
        11.1%
        D9_1_2_R1
        15.8
        13.4
        160
        1.19
        1.15
        0.18%
        0.0
        15.8
        99.7%
        15.8
        0.16%
        0.0
        13.4
        100.0%
        13.4
        100%
        D9_1_2_R1_T1
        15.8
        4.8%
        48%
        50 bp
        15.8
        1.1%
        4.6%
        49%
        49 bp
        15.8
        0.18%
        0.0
        15.8
        99.7%
        15.8
        3.6%
        D9_2_160_R1
        76.4
        45.3
        195
        1.02
        0.94
        0.32%
        0.0
        76.4
        99.1%
        77.1
        0.25%
        0.0
        45.3
        100.0%
        45.3
        100%
        D9_2_160_R1_T1
        76.4
        30.7%
        45%
        50 bp
        77.1
        1.2%
        29.2%
        45%
        49 bp
        77.1
        0.32%
        0.0
        76.4
        99.1%
        77.1
        29.6%
        D9_2_27_R1
        53.3
        43.7
        90
        1.01
        0.82
        0.34%
        0.0
        53.3
        89.4%
        59.6
        0.22%
        0.0
        43.7
        100.0%
        43.7
        100%
        D9_2_27_R1_T1
        53.3
        5.9%
        41%
        150 bp
        59.6
        58.6%
        4.6%
        42%
        62 bp
        59.6
        0.34%
        0.0
        53.3
        89.4%
        59.6
        4.6%
        D9_2_304_R1
        23.4
        16.3
        150
        1.14
        1.27
        0.15%
        0.0
        23.4
        98.3%
        23.8
        0.14%
        0.0
        16.3
        100.0%
        16.3
        100%
        D9_2_304_R1_T1
        23.4
        18.8%
        42%
        37 bp
        23.8
        1.5%
        18.5%
        42%
        36 bp
        23.8
        0.15%
        0.0
        23.4
        98.3%
        23.8
        14.8%
        D9_3_153_R1
        30.3
        18.5
        180
        3.73
        1.64
        0.21%
        0.0
        30.3
        99.4%
        30.5
        0.18%
        0.0
        18.5
        100.0%
        18.5
        100%
        D9_3_153_R1_T1
        30.3
        30.9%
        44%
        50 bp
        30.5
        1.2%
        29.5%
        45%
        49 bp
        30.5
        0.21%
        0.0
        30.3
        99.4%
        30.5
        31.1%
        D9_3_157_R1
        44.2
        35.4
        175
        1.30
        1.14
        0.29%
        0.0
        44.2
        99.5%
        44.5
        0.27%
        0.0
        35.4
        100.0%
        35.4
        100%
        D9_3_157_R1_T1
        44.2
        11.5%
        48%
        50 bp
        44.5
        1.2%
        10.8%
        48%
        49 bp
        44.5
        0.29%
        0.0
        44.2
        99.5%
        44.5
        11.4%
        D9_4_353_R1
        42.6
        37.5
        170
        1.11
        1.15
        0.23%
        0.0
        42.6
        99.8%
        42.7
        0.21%
        0.0
        37.5
        100.0%
        37.5
        100%
        D9_4_353_R1_T1
        42.6
        3.7%
        46%
        50 bp
        42.7
        1.1%
        3.6%
        46%
        49 bp
        42.7
        0.23%
        0.0
        42.6
        99.8%
        42.7
        2.5%
        D9_5_354_R1
        37.1
        32.2
        175
        1.18
        1.14
        0.24%
        0.0
        37.1
        99.8%
        37.2
        0.22%
        0.0
        32.2
        100.0%
        32.2
        100%
        D9_5_354_R1_T1
        37.1
        4.5%
        47%
        50 bp
        37.2
        1.0%
        4.4%
        47%
        49 bp
        37.2
        0.24%
        0.0
        37.1
        99.8%
        37.2
        3.5%
        DFAM71927V3_1_739_R1
        50.8
        2.4
        110
        1.25
        0.75
        0.48%
        0.0
        50.8
        80.2%
        63.3
        0.23%
        0.0
        2.4
        100.0%
        2.4
        100%
        DFAM71927V3_1_739_R1_T1
        50.8
        82.0%
        43%
        75 bp
        63.4
        1.5%
        80.3%
        43%
        73 bp
        63.3
        0.48%
        0.0
        50.8
        80.2%
        63.3
        91.9%
        DFAM71927V3_1_741_R1
        51.5
        9.3
        165
        1.95
        1.24
        0.50%
        0.0
        51.5
        84.9%
        60.6
        0.24%
        0.0
        9.3
        100.0%
        9.3
        100%
        DFAM71927V3_1_741_R1_T1
        51.5
        67.1%
        43%
        75 bp
        60.7
        1.3%
        64.5%
        43%
        73 bp
        60.6
        0.50%
        0.0
        51.5
        84.9%
        60.6
        77.2%
        DFAM71927V3_1_742_R1
        48.9
        4.3
        155
        1.67
        1.07
        0.49%
        0.0
        48.9
        81.7%
        59.8
        0.23%
        0.0
        4.3
        100.0%
        4.3
        100%
        DFAM71927V3_1_742_R1_T1
        48.9
        77.6%
        45%
        75 bp
        59.8
        1.5%
        75.4%
        45%
        73 bp
        59.8
        0.49%
        0.0
        48.9
        81.7%
        59.8
        87.3%
        DFAM71927V3_1_743_R1
        81.2
        4.3
        150
        1.88
        1.12
        0.51%
        0.0
        81.2
        86.2%
        94.2
        0.25%
        0.0
        4.3
        100.0%
        4.3
        100%
        DFAM71927V3_1_743_R1_T1
        81.2
        81.1%
        46%
        75 bp
        94.4
        1.5%
        79.3%
        46%
        73 bp
        94.2
        0.51%
        0.0
        81.2
        86.2%
        94.2
        91.6%
        DFAM71927V3_1_745_R1
        55.0
        6.5
        125
        1.02
        0.27
        0.44%
        0.0
        55.0
        62.3%
        88.3
        0.22%
        0.0
        6.5
        100.0%
        6.5
        100%
        DFAM71927V3_1_745_R1_T1
        55.0
        79.6%
        41%
        75 bp
        88.4
        1.2%
        77.7%
        41%
        73 bp
        88.3
        0.44%
        0.0
        55.0
        62.3%
        88.3
        83.1%
        DFAM71927V3_1_748_R1
        58.4
        3.1
        140
        3.11
        1.19
        0.39%
        0.0
        58.4
        83.8%
        69.8
        0.21%
        0.0
        3.1
        100.0%
        3.1
        100%
        DFAM71927V3_1_748_R1_T1
        58.4
        85.3%
        46%
        75 bp
        69.8
        1.4%
        83.6%
        46%
        73 bp
        69.8
        0.39%
        0.0
        58.4
        83.8%
        69.8
        91.8%
        DFAM71927V3_1_750_R1
        82.0
        1.2
        110
        1.30
        0.59
        0.38%
        0.0
        82.0
        77.4%
        106.0
        0.26%
        0.0
        1.2
        100.0%
        1.2
        100%
        DFAM71927V3_1_750_R1_T1
        82.0
        91.7%
        43%
        75 bp
        106.0
        1.6%
        90.9%
        43%
        73 bp
        106.0
        0.38%
        0.0
        82.0
        77.4%
        106.0
        96.1%
        DFAM71927V3_1_751_R1
        78.2
        13.1
        160
        1.85
        1.22
        0.38%
        0.0
        78.2
        88.5%
        88.3
        0.20%
        0.0
        13.1
        100.0%
        13.1
        100%
        DFAM71927V3_1_751_R1_T1
        78.2
        73.7%
        46%
        75 bp
        88.3
        1.3%
        71.7%
        46%
        73 bp
        88.3
        0.38%
        0.0
        78.2
        88.5%
        88.3
        78.8%
        HEL9217_1_593_R1
        25.0
        21.7
        165
        1.19
        1.13
        0.18%
        0.0
        25.0
        99.8%
        25.1
        0.16%
        0.0
        21.7
        100.0%
        21.7
        100%
        HEL9217_1_593_R1_T1
        25.0
        4.7%
        46%
        50 bp
        25.1
        1.1%
        4.5%
        46%
        49 bp
        25.1
        0.18%
        0.0
        25.0
        99.8%
        25.1
        3.5%
        HEL9217_2_594_R1
        34.3
        29.6
        160
        1.17
        1.13
        0.18%
        0.0
        34.3
        99.7%
        34.4
        0.16%
        0.0
        29.6
        100.0%
        29.6
        100%
        HEL9217_2_594_R1_T1
        34.3
        5.5%
        46%
        50 bp
        34.4
        1.1%
        5.2%
        46%
        49 bp
        34.4
        0.18%
        0.0
        34.3
        99.7%
        34.4
        4.2%
        HL60_1_53_R1
        29.5
        25.4
        120
        1.21
        0.99
        0.18%
        0.0
        29.5
        99.1%
        29.8
        0.16%
        0.0
        25.4
        100.0%
        25.4
        100%
        HL60_1_53_R1_T1
        29.5
        4.6%
        47%
        50 bp
        29.8
        2.2%
        4.5%
        47%
        49 bp
        29.8
        0.18%
        0.0
        29.5
        99.1%
        29.8
        3.7%
        HL60_2_77_R1
        27.1
        23.8
        140
        1.06
        0.94
        0.19%
        0.0
        27.1
        99.6%
        27.2
        0.17%
        0.0
        23.8
        100.0%
        23.8
        100%
        HL60_2_77_R1_T1
        27.1
        3.2%
        47%
        50 bp
        27.2
        1.5%
        3.1%
        48%
        49 bp
        27.2
        0.19%
        0.0
        27.1
        99.6%
        27.2
        2.3%
        IGG_D9_4_R1
        19.6
        14.9
        0
        1.00
        0.19
        0.21%
        0.0
        19.6
        99.5%
        19.7
        0.16%
        0.0
        14.9
        100.0%
        14.9
        100%
        IGG_D9_4_R1_T1
        19.6
        13.5%
        41%
        50 bp
        19.7
        1.1%
        13.3%
        41%
        49 bp
        19.7
        0.21%
        0.0
        19.6
        99.5%
        19.7
        10.9%
        IGG_M6_R1
        37.6
        27.8
        125
        1.00
        0.33
        0.25%
        0.0
        37.6
        98.6%
        38.2
        0.20%
        0.0
        27.8
        100.0%
        27.8
        100%
        IGG_M6_R1_T1
        37.6
        15.5%
        41%
        50 bp
        38.2
        1.3%
        15.0%
        41%
        49 bp
        38.2
        0.25%
        0.0
        37.6
        98.6%
        38.2
        12.8%
        IGG_MV411_R1
        35.1
        26.2
        0
        1.00
        0.32
        0.24%
        0.0
        35.1
        99.5%
        35.3
        0.19%
        0.0
        26.2
        100.0%
        26.2
        100%
        IGG_MV411_R1_T1
        35.1
        15.6%
        39%
        50 bp
        35.3
        1.2%
        14.8%
        40%
        49 bp
        35.3
        0.24%
        0.0
        35.1
        99.5%
        35.3
        13.0%
        INPUT_D0_R1
        81.2
        63.8
        0
        1.00
        0.33
        0.32%
        0.0
        81.2
        99.3%
        81.7
        0.27%
        0.0
        63.8
        100.0%
        63.8
        100%
        6.5%
        INPUT_D0_R1_T1
        27.9
        8.0%
        40%
        50 bp
        28.2
        2.4%
        7.7%
        40%
        49 bp
        28.2
        0.43%
        0.0
        27.9
        99.0%
        28.2
        INPUT_D0_R1_T2
        15.6
        4.2%
        41%
        50 bp
        15.7
        1.1%
        4.1%
        41%
        49 bp
        15.7
        0.21%
        0.0
        15.6
        99.6%
        15.7
        INPUT_D0_R1_T3
        37.7
        14.4%
        42%
        50 bp
        37.9
        1.7%
        13.8%
        42%
        49 bp
        37.9
        0.28%
        0.0
        37.7
        99.4%
        37.9
        INPUT_D9_R1
        89.3
        74.2
        120
        1.00
        1.02
        0.31%
        0.0
        89.3
        99.6%
        89.7
        0.18%
        0.0
        74.2
        100.0%
        74.2
        100%
        1.3%
        INPUT_D9_R1_T1
        35.6
        12.5%
        42%
        50 bp
        35.9
        1.7%
        12.0%
        42%
        49 bp
        35.8
        0.27%
        0.0
        35.6
        99.4%
        35.8
        INPUT_D9_R1_T2
        39.7
        5.9%
        41%
        150 bp
        39.8
        20.2%
        5.3%
        41%
        119 bp
        39.7
        0.34%
        0.0
        39.7
        99.8%
        39.7
        INPUT_D9_R1_T3
        14.0
        3.5%
        41%
        50 bp
        14.1
        1.1%
        3.5%
        42%
        49 bp
        14.1
        0.20%
        0.0
        14.0
        99.6%
        14.1
        INPUT_DFAM71927V3_R1
        76.2
        67.5
        0
        1.00
        0.45
        0.40%
        0.0
        76.2
        98.2%
        77.6
        0.27%
        0.0
        67.5
        100.0%
        67.5
        100%
        2.5%
        INPUT_DFAM71927V3_R1_T1
        39.9
        4.6%
        40%
        100 bp
        40.6
        1.4%
        4.5%
        40%
        98 bp
        40.6
        0.34%
        0.0
        39.9
        98.3%
        40.6
        INPUT_DFAM71927V3_R1_T2
        36.3
        4.0%
        39%
        75 bp
        37.1
        1.0%
        3.8%
        40%
        74 bp
        37.1
        0.49%
        0.0
        36.3
        98.0%
        37.1
        INPUT_HEL9217_R1
        32.9
        27.8
        0
        1.00
        0.31
        0.22%
        0.0
        32.9
        99.6%
        33.0
        0.17%
        0.0
        27.8
        100.0%
        27.8
        100%
        INPUT_HEL9217_R1_T1
        32.9
        4.9%
        41%
        50 bp
        33.0
        1.1%
        4.8%
        41%
        49 bp
        33.0
        0.22%
        0.0
        32.9
        99.6%
        33.0
        2.2%
        INPUT_HL60_R1
        25.8
        14.5
        130
        1.01
        0.37
        0.21%
        0.0
        25.8
        99.5%
        25.9
        0.15%
        0.0
        14.5
        100.0%
        14.5
        100%
        INPUT_HL60_R1_T1
        25.8
        34.8%
        43%
        50 bp
        25.9
        1.2%
        33.6%
        44%
        49 bp
        25.9
        0.21%
        0.0
        25.8
        99.5%
        25.9
        31.6%
        INPUT_KG1_R1
        33.9
        28.8
        0
        1.01
        0.42
        0.35%
        0.0
        33.9
        99.2%
        34.2
        0.30%
        0.0
        28.8
        100.0%
        28.8
        100%
        INPUT_KG1_R1_T1
        33.9
        4.8%
        39%
        50 bp
        34.2
        1.0%
        4.6%
        39%
        49 bp
        34.2
        0.35%
        0.0
        33.9
        99.2%
        34.2
        2.5%
        INPUT_Kasumi1_R1
        31.1
        26.3
        0
        1.00
        0.38
        0.33%
        0.0
        31.1
        99.3%
        31.4
        0.28%
        0.0
        26.3
        100.0%
        26.3
        100%
        INPUT_Kasumi1_R1_T1
        31.1
        5.1%
        39%
        50 bp
        31.4
        1.2%
        5.0%
        40%
        49 bp
        31.4
        0.33%
        0.0
        31.1
        99.3%
        31.4
        2.5%
        INPUT_M6_R1
        16.2
        13.5
        135
        1.00
        0.19
        0.25%
        0.0
        16.2
        99.4%
        16.3
        0.20%
        0.0
        13.5
        100.0%
        13.5
        100%
        INPUT_M6_R1_T1
        16.2
        4.4%
        42%
        50 bp
        16.3
        1.7%
        4.3%
        42%
        49 bp
        16.3
        0.25%
        0.0
        16.2
        99.4%
        16.3
        2.1%
        INPUT_MOLM13_R1
        37.7
        32.0
        0
        1.01
        0.65
        0.32%
        0.0
        37.7
        99.3%
        37.9
        0.28%
        0.0
        32.0
        100.0%
        32.0
        100%
        INPUT_MOLM13_R1_T1
        37.7
        5.5%
        39%
        50 bp
        38.0
        1.2%
        5.4%
        40%
        49 bp
        37.9
        0.32%
        0.0
        37.7
        99.3%
        37.9
        3.2%
        INPUT_MONOMAC1_R1
        28.0
        23.8
        0
        1.00
        0.32
        0.21%
        0.0
        28.0
        99.6%
        28.1
        0.17%
        0.0
        23.8
        100.0%
        23.8
        100%
        INPUT_MONOMAC1_R1_T1
        28.0
        4.5%
        41%
        50 bp
        28.1
        1.1%
        4.4%
        41%
        49 bp
        28.1
        0.21%
        0.0
        28.0
        99.6%
        28.1
        1.7%
        INPUT_MONOMAC6_R1
        32.0
        26.5
        140
        1.01
        0.62
        0.33%
        0.0
        32.0
        99.2%
        32.2
        0.28%
        0.0
        26.5
        100.0%
        26.5
        100%
        INPUT_MONOMAC6_R1_T1
        32.0
        5.6%
        42%
        50 bp
        32.2
        1.2%
        5.5%
        43%
        49 bp
        32.2
        0.33%
        0.0
        32.0
        99.2%
        32.2
        2.0%
        INPUT_MV411_R1
        41.7
        34.5
        110
        1.00
        0.35
        0.23%
        0.0
        41.7
        99.5%
        42.0
        0.19%
        0.0
        34.5
        100.0%
        34.5
        100%
        INPUT_MV411_R1_T1
        41.7
        6.5%
        42%
        50 bp
        42.0
        1.6%
        6.3%
        43%
        49 bp
        42.0
        0.23%
        0.0
        41.7
        99.5%
        42.0
        3.5%
        INPUT_NB4_R1
        28.5
        23.7
        155
        1.01
        0.50
        0.34%
        0.0
        28.5
        99.2%
        28.8
        0.28%
        0.0
        23.7
        100.0%
        23.7
        100%
        INPUT_NB4_R1_T1
        28.5
        4.9%
        43%
        50 bp
        28.8
        1.2%
        4.8%
        43%
        49 bp
        28.8
        0.34%
        0.0
        28.5
        99.2%
        28.8
        2.1%
        INPUT_NOMO1_R1
        20.2
        17.1
        95
        1.00
        0.30
        0.23%
        0.0
        20.2
        99.5%
        20.3
        0.19%
        0.0
        17.1
        100.0%
        17.1
        100%
        INPUT_NOMO1_R1_T1
        20.2
        4.2%
        42%
        50 bp
        20.3
        1.7%
        4.1%
        42%
        49 bp
        20.3
        0.23%
        0.0
        20.2
        99.5%
        20.3
        1.7%
        INPUT_OCIAML2_R1
        73.5
        62.7
        150
        1.00
        1.04
        0.34%
        0.0
        73.5
        99.5%
        73.8
        0.19%
        0.0
        62.7
        100.0%
        62.7
        100%
        3.7%
        INPUT_OCIAML2_R1_T1
        27.1
        8.1%
        41%
        50 bp
        27.4
        1.9%
        7.8%
        41%
        49 bp
        27.4
        0.31%
        0.0
        27.1
        99.1%
        27.4
        INPUT_OCIAML2_R1_T2
        46.3
        6.1%
        42%
        150 bp
        46.4
        21.6%
        5.3%
        41%
        117 bp
        46.4
        0.35%
        0.0
        46.3
        99.8%
        46.4
        INPUT_OCIAML3_R1
        29.8
        24.7
        165
        1.01
        0.69
        0.31%
        0.0
        29.8
        99.2%
        30.0
        0.27%
        0.0
        24.7
        100.0%
        24.7
        100%
        INPUT_OCIAML3_R1_T1
        29.8
        5.3%
        42%
        50 bp
        30.0
        1.1%
        5.2%
        42%
        49 bp
        30.0
        0.31%
        0.0
        29.8
        99.2%
        30.0
        2.1%
        INPUT_P31FUJ_R1
        32.8
        27.5
        0
        1.00
        0.30
        0.23%
        0.0
        32.8
        99.6%
        32.9
        0.18%
        0.0
        27.5
        100.0%
        27.5
        100%
        INPUT_P31FUJ_R1_T1
        32.8
        5.3%
        41%
        50 bp
        32.9
        1.1%
        5.2%
        41%
        49 bp
        32.9
        0.23%
        0.0
        32.8
        99.6%
        32.9
        2.3%
        INPUT_PLB985_R1
        49.6
        41.8
        0
        1.00
        0.35
        0.22%
        0.0
        49.6
        99.6%
        49.8
        0.17%
        0.0
        41.8
        100.0%
        41.8
        100%
        INPUT_PLB985_R1_T1
        49.6
        5.4%
        41%
        50 bp
        49.8
        1.1%
        5.3%
        41%
        49 bp
        49.8
        0.22%
        0.0
        49.6
        99.6%
        49.8
        2.5%
        INPUT_SKNO1_R1
        32.2
        26.4
        155
        1.01
        0.62
        0.33%
        0.0
        32.2
        99.2%
        32.5
        0.28%
        0.0
        26.4
        100.0%
        26.4
        100%
        INPUT_SKNO1_R1_T1
        32.2
        5.6%
        43%
        50 bp
        32.5
        1.2%
        5.5%
        43%
        49 bp
        32.5
        0.33%
        0.0
        32.2
        99.2%
        32.5
        2.4%
        INPUT_TF1_R1
        24.2
        20.0
        165
        1.01
        0.62
        0.33%
        0.0
        24.2
        99.1%
        24.5
        0.28%
        0.0
        20.0
        100.0%
        20.0
        100%
        INPUT_TF1_R1_T1
        24.2
        5.5%
        43%
        50 bp
        24.5
        1.2%
        5.3%
        43%
        49 bp
        24.5
        0.33%
        0.0
        24.2
        99.1%
        24.5
        2.5%
        INPUT_U937_R1
        18.2
        15.3
        120
        1.01
        0.30
        0.23%
        0.0
        18.2
        99.4%
        18.3
        0.19%
        0.0
        15.3
        100.0%
        15.3
        100%
        INPUT_U937_R1_T1
        18.2
        4.4%
        42%
        50 bp
        18.3
        1.7%
        4.3%
        43%
        49 bp
        18.3
        0.23%
        0.0
        18.2
        99.4%
        18.3
        1.9%
        INPUT_UCSDAML1_R1
        21.3
        16.8
        110
        1.01
        0.33
        0.20%
        0.0
        21.3
        99.6%
        21.4
        0.16%
        0.0
        16.8
        100.0%
        16.8
        100%
        INPUT_UCSDAML1_R1_T1
        21.3
        10.8%
        42%
        50 bp
        21.4
        1.2%
        10.4%
        42%
        49 bp
        21.4
        0.20%
        0.0
        21.3
        99.6%
        21.4
        8.3%
        INPUT_UT7_R1
        21.0
        17.5
        95
        1.01
        0.31
        0.24%
        0.0
        21.0
        99.4%
        21.1
        0.20%
        0.0
        17.5
        100.0%
        17.5
        100%
        INPUT_UT7_R1_T1
        21.0
        4.8%
        43%
        50 bp
        21.1
        1.7%
        4.7%
        43%
        49 bp
        21.1
        0.24%
        0.0
        21.0
        99.4%
        21.1
        2.3%
        KG1_1_58_R1
        34.7
        30.1
        125
        1.09
        0.96
        0.21%
        0.0
        34.7
        99.2%
        34.9
        0.19%
        0.0
        30.1
        100.0%
        30.1
        100%
        KG1_1_58_R1_T1
        34.7
        3.4%
        46%
        50 bp
        34.9
        2.0%
        3.3%
        47%
        49 bp
        34.9
        0.21%
        0.0
        34.7
        99.2%
        34.9
        2.2%
        KG1_2_207_R1
        23.4
        20.3
        160
        1.20
        1.12
        0.18%
        0.0
        23.4
        99.8%
        23.5
        0.16%
        0.0
        20.3
        100.0%
        20.3
        100%
        KG1_2_207_R1_T1
        23.4
        5.4%
        47%
        50 bp
        23.5
        1.2%
        5.1%
        47%
        49 bp
        23.5
        0.18%
        0.0
        23.4
        99.8%
        23.5
        4.5%
        Kasumi1_1_56_R1
        37.1
        31.4
        125
        1.25
        1.01
        0.18%
        0.0
        37.1
        99.2%
        37.4
        0.16%
        0.0
        31.4
        100.0%
        31.4
        100%
        Kasumi1_1_56_R1_T1
        37.1
        5.8%
        48%
        50 bp
        37.4
        2.0%
        5.7%
        49%
        49 bp
        37.4
        0.18%
        0.0
        37.1
        99.2%
        37.4
        4.7%
        M6_1_109_R1
        25.8
        14.4
        155
        1.31
        1.61
        0.22%
        0.0
        25.8
        99.2%
        26.0
        0.17%
        0.0
        14.4
        100.0%
        14.4
        100%
        M6_1_109_R1_T1
        25.8
        35.0%
        42%
        50 bp
        26.0
        1.2%
        34.2%
        42%
        49 bp
        26.0
        0.22%
        0.0
        25.8
        99.2%
        26.0
        33.0%
        M6_1_123_R1
        56.2
        42.5
        185
        1.04
        1.15
        0.22%
        0.0
        56.2
        99.5%
        56.5
        0.17%
        0.0
        42.5
        100.0%
        42.5
        100%
        M6_1_123_R1_T1
        56.2
        15.3%
        41%
        50 bp
        56.5
        1.1%
        15.0%
        41%
        49 bp
        56.5
        0.22%
        0.0
        56.2
        99.5%
        56.5
        12.7%
        M6_1_124_R1
        31.8
        26.2
        165
        1.01
        0.69
        0.23%
        0.0
        31.8
        99.4%
        32.0
        0.18%
        0.0
        26.2
        100.0%
        26.2
        100%
        M6_1_124_R1_T1
        31.8
        7.7%
        40%
        50 bp
        32.0
        1.1%
        7.6%
        40%
        49 bp
        32.0
        0.23%
        0.0
        31.8
        99.4%
        32.0
        5.3%
        M6_1_125_R1
        22.1
        16.9
        160
        1.02
        0.75
        0.23%
        0.0
        22.1
        99.3%
        22.3
        0.17%
        0.0
        16.9
        100.0%
        16.9
        100%
        M6_1_125_R1_T1
        22.1
        13.3%
        40%
        50 bp
        22.3
        1.1%
        13.2%
        41%
        49 bp
        22.3
        0.23%
        0.0
        22.1
        99.3%
        22.3
        11.1%
        M6_1_127_R1
        26.1
        21.0
        185
        1.04
        1.03
        0.22%
        0.0
        26.1
        98.5%
        26.5
        0.18%
        0.0
        21.0
        100.0%
        21.0
        100%
        M6_1_127_R1_T1
        26.1
        8.9%
        42%
        50 bp
        26.5
        1.4%
        8.8%
        42%
        49 bp
        26.5
        0.22%
        0.0
        26.1
        98.5%
        26.5
        6.7%
        M6_1_128_R1
        26.6
        21.6
        185
        1.02
        0.78
        0.22%
        0.0
        26.6
        98.8%
        27.0
        0.18%
        0.0
        21.6
        100.0%
        21.6
        100%
        M6_1_128_R1_T1
        26.6
        8.5%
        41%
        50 bp
        27.0
        1.2%
        8.4%
        41%
        49 bp
        27.0
        0.22%
        0.0
        26.6
        98.8%
        27.0
        6.2%
        M6_1_171_R1
        39.0
        25.6
        140
        1.03
        1.03
        0.27%
        0.0
        39.0
        99.4%
        39.2
        0.22%
        0.0
        25.6
        100.0%
        25.6
        100%
        M6_1_171_R1_T1
        39.0
        25.2%
        40%
        50 bp
        39.2
        1.2%
        24.1%
        40%
        49 bp
        39.2
        0.27%
        0.0
        39.0
        99.4%
        39.2
        23.5%
        M6_2_122_R1
        25.1
        20.6
        160
        1.58
        1.61
        0.21%
        0.0
        25.1
        99.4%
        25.2
        0.17%
        0.0
        20.6
        100.0%
        20.6
        100%
        M6_2_122_R1_T1
        25.1
        8.7%
        41%
        50 bp
        25.2
        1.1%
        8.5%
        41%
        49 bp
        25.2
        0.21%
        0.0
        25.1
        99.4%
        25.2
        7.0%
        M6_2_302_R1
        19.9
        13.3
        175
        1.34
        1.75
        0.16%
        0.0
        19.9
        97.3%
        20.5
        0.14%
        0.0
        13.3
        100.0%
        13.3
        100%
        M6_2_302_R1_T1
        19.9
        21.7%
        43%
        37 bp
        20.5
        1.7%
        21.2%
        43%
        36 bp
        20.5
        0.16%
        0.0
        19.9
        97.3%
        20.5
        18.1%
        M6_3_355_R1
        35.0
        29.5
        185
        1.32
        1.15
        0.23%
        0.0
        35.0
        99.8%
        35.0
        0.22%
        0.0
        29.5
        100.0%
        29.5
        100%
        M6_3_355_R1_T1
        35.0
        7.1%
        49%
        50 bp
        35.0
        1.0%
        6.9%
        49%
        49 bp
        35.0
        0.23%
        0.0
        35.0
        99.8%
        35.0
        6.6%
        M6_4_356_R1
        29.0
        25.6
        180
        1.08
        1.15
        0.23%
        0.0
        29.0
        99.8%
        29.1
        0.22%
        0.0
        25.6
        100.0%
        25.6
        100%
        M6_4_356_R1_T1
        29.0
        2.6%
        45%
        50 bp
        29.1
        1.1%
        2.6%
        45%
        49 bp
        29.1
        0.23%
        0.0
        29.0
        99.8%
        29.1
        1.5%
        MOLM13_1_48_R1
        32.9
        28.3
        105
        1.18
        0.97
        0.18%
        0.0
        32.9
        99.0%
        33.2
        0.16%
        0.0
        28.3
        100.0%
        28.3
        100%
        MOLM13_1_48_R1_T1
        32.9
        4.5%
        48%
        50 bp
        33.2
        2.4%
        4.4%
        48%
        49 bp
        33.2
        0.18%
        0.0
        32.9
        99.0%
        33.2
        3.5%
        MONOMAC1_1_591_R1
        56.6
        44.7
        175
        1.28
        1.12
        0.18%
        0.0
        56.6
        99.7%
        56.7
        0.16%
        0.0
        44.7
        100.0%
        44.7
        100%
        MONOMAC1_1_591_R1_T1
        56.6
        12.7%
        48%
        50 bp
        56.7
        1.1%
        12.0%
        49%
        49 bp
        56.7
        0.18%
        0.0
        56.6
        99.7%
        56.7
        11.3%
        MONOMAC1_2_592_R1
        34.4
        28.6
        165
        1.33
        1.15
        0.17%
        0.0
        34.4
        99.7%
        34.5
        0.16%
        0.0
        28.6
        100.0%
        28.6
        100%
        MONOMAC1_2_592_R1_T1
        34.4
        8.4%
        48%
        50 bp
        34.5
        1.1%
        8.0%
        48%
        49 bp
        34.5
        0.17%
        0.0
        34.4
        99.7%
        34.5
        7.2%
        MONOMAC1_3_777_R1
        46.5
        40.9
        255
        1.04
        1.22
        0.36%
        0.0
        46.5
        99.6%
        46.7
        0.26%
        0.0
        40.9
        100.0%
        40.9
        100%
        MONOMAC1_3_777_R1_T1
        46.5
        3.8%
        41%
        75 bp
        46.7
        0.9%
        3.6%
        41%
        74 bp
        46.7
        0.36%
        0.0
        46.5
        99.6%
        46.7
        4.4%
        MONOMAC1_4_778_R1
        55.2
        48.0
        295
        1.03
        1.19
        0.39%
        0.0
        55.2
        99.5%
        55.5
        0.26%
        0.0
        48.0
        100.0%
        48.0
        100%
        MONOMAC1_4_778_R1_T1
        55.2
        4.6%
        39%
        75 bp
        55.5
        0.9%
        4.5%
        39%
        74 bp
        55.5
        0.39%
        0.0
        55.2
        99.5%
        55.5
        5.2%
        MONOMAC6_1_196_R1
        16.2
        11.2
        180
        1.46
        1.24
        0.18%
        0.0
        16.2
        99.8%
        16.3
        0.16%
        0.0
        11.2
        100.0%
        11.2
        100%
        MONOMAC6_1_196_R1_T1
        16.2
        24.4%
        50%
        50 bp
        16.5
        2.1%
        23.2%
        50%
        50 bp
        16.3
        0.18%
        0.0
        16.2
        99.8%
        16.3
        22.6%
        MONOMAC6_2_206_R1
        18.6
        15.3
        195
        1.16
        1.30
        0.17%
        0.0
        18.6
        99.8%
        18.6
        0.15%
        0.0
        15.3
        100.0%
        15.3
        100%
        MONOMAC6_2_206_R1_T1
        18.6
        8.9%
        45%
        50 bp
        18.6
        1.3%
        8.5%
        45%
        49 bp
        18.6
        0.17%
        0.0
        18.6
        99.8%
        18.6
        7.9%
        MV411-DMSO-8h_1_728_R1
        61.4
        8.6
        115
        1.26
        1.04
        0.31%
        0.0
        61.4
        92.4%
        66.4
        0.18%
        0.0
        8.6
        100.0%
        8.6
        100%
        MV411-DMSO-8h_1_728_R1_T1
        61.4
        79.2%
        43%
        75 bp
        66.4
        1.4%
        77.9%
        43%
        73 bp
        66.4
        0.31%
        0.0
        61.4
        92.4%
        66.4
        80.9%
        MV411-DMSO-8h_2_724_R1
        50.7
        8.9
        150
        1.25
        1.05
        0.47%
        0.0
        50.7
        95.6%
        53.0
        0.23%
        0.0
        8.9
        100.0%
        8.9
        100%
        MV411-DMSO-8h_2_724_R1_T1
        50.7
        70.3%
        45%
        75 bp
        53.0
        2.8%
        68.5%
        45%
        72 bp
        53.0
        0.47%
        0.0
        50.7
        95.6%
        53.0
        77.1%
        MV411-DMSO-8h_2_734_R1
        66.4
        48.2
        160
        1.37
        1.05
        0.44%
        0.0
        66.4
        99.2%
        66.9
        0.32%
        0.0
        48.2
        100.0%
        48.2
        100%
        MV411-DMSO-8h_2_734_R1_T1
        66.4
        17.3%
        49%
        75 bp
        66.9
        1.3%
        16.5%
        49%
        73 bp
        66.9
        0.44%
        0.0
        66.4
        99.2%
        66.9
        21.4%
        MV411-DMSO_1_183_R1
        16.7
        14.3
        160
        1.27
        1.16
        0.19%
        0.0
        16.7
        99.7%
        16.8
        0.17%
        0.0
        14.3
        100.0%
        14.3
        100%
        MV411-DMSO_1_183_R1_T1
        16.7
        5.2%
        47%
        50 bp
        16.8
        1.0%
        5.1%
        48%
        50 bp
        16.8
        0.19%
        0.0
        16.7
        99.7%
        16.8
        3.8%
        MV411-IRF2BP2-CT-GFP-8_1_762_R1
        164.9
        121.2
        230
        1.01
        1.12
        0.36%
        0.0
        164.9
        99.3%
        166.1
        0.24%
        0.0
        121.2
        100.0%
        121.2
        100%
        MV411-IRF2BP2-CT-GFP-8_1_762_R1_T1
        164.9
        17.6%
        41%
        75 bp
        166.1
        0.9%
        16.9%
        41%
        74 bp
        166.1
        0.36%
        0.0
        164.9
        99.3%
        166.1
        18.6%
        MV411-MEF2D-NT-SC-63_1_760_R1
        74.2
        49.3
        235
        1.03
        1.49
        0.36%
        0.0
        74.2
        97.5%
        76.1
        0.23%
        0.0
        49.3
        100.0%
        49.3
        100%
        MV411-MEF2D-NT-SC-63_1_760_R1_T1
        74.2
        24.2%
        41%
        75 bp
        76.2
        1.0%
        23.2%
        41%
        74 bp
        76.1
        0.36%
        0.0
        74.2
        97.5%
        76.1
        25.7%
        MV411-PU1-NT-SC-1_1_761_R1
        83.8
        62.4
        240
        1.71
        1.71
        0.32%
        0.0
        83.8
        99.5%
        84.2
        0.24%
        0.0
        62.4
        100.0%
        62.4
        100%
        MV411-PU1-NT-SC-1_1_761_R1_T1
        83.8
        17.5%
        43%
        75 bp
        84.5
        1.3%
        16.8%
        43%
        74 bp
        84.2
        0.32%
        0.0
        83.8
        99.5%
        84.2
        19.0%
        MV411_1_106_R1
        39.9
        21.0
        175
        1.10
        1.56
        0.22%
        0.0
        39.9
        99.4%
        40.2
        0.17%
        0.0
        21.0
        100.0%
        21.0
        100%
        MV411_1_106_R1_T1
        39.9
        38.7%
        43%
        50 bp
        40.2
        1.1%
        37.8%
        43%
        49 bp
        40.2
        0.22%
        0.0
        39.9
        99.4%
        40.2
        35.7%
        MV411_1_115_R1
        19.9
        14.0
        150
        1.03
        0.79
        0.24%
        0.0
        19.9
        99.4%
        20.0
        0.19%
        0.0
        14.0
        100.0%
        14.0
        100%
        MV411_1_115_R1_T1
        19.9
        18.5%
        42%
        50 bp
        20.0
        1.1%
        17.9%
        42%
        49 bp
        20.0
        0.24%
        0.0
        19.9
        99.4%
        20.0
        15.9%
        MV411_1_117_R1
        27.0
        18.9
        135
        1.02
        0.66
        0.22%
        0.0
        27.0
        99.4%
        27.2
        0.17%
        0.0
        18.9
        100.0%
        18.9
        100%
        MV411_1_117_R1_T1
        27.0
        19.1%
        44%
        50 bp
        27.2
        1.1%
        18.4%
        44%
        49 bp
        27.2
        0.22%
        0.0
        27.0
        99.4%
        27.2
        16.0%
        MV411_1_118_R1
        31.0
        24.0
        165
        1.01
        0.61
        0.23%
        0.0
        31.0
        99.6%
        31.2
        0.18%
        0.0
        24.0
        100.0%
        24.0
        100%
        MV411_1_118_R1_T1
        31.0
        11.8%
        43%
        50 bp
        31.2
        1.2%
        11.4%
        43%
        49 bp
        31.2
        0.23%
        0.0
        31.0
        99.6%
        31.2
        8.6%
        MV411_1_137_R1
        40.8
        33.8
        220
        1.02
        0.74
        0.22%
        0.0
        40.8
        99.7%
        41.0
        0.19%
        0.0
        33.8
        100.0%
        33.8
        100%
        MV411_1_137_R1_T1
        40.8
        7.7%
        41%
        50 bp
        41.0
        1.3%
        7.3%
        41%
        49 bp
        41.0
        0.22%
        0.0
        40.8
        99.7%
        41.0
        5.4%
        MV411_1_138_R1
        34.5
        25.4
        190
        1.52
        1.11
        0.24%
        0.0
        34.5
        99.4%
        34.7
        0.20%
        0.0
        25.4
        100.0%
        25.4
        100%
        MV411_1_138_R1_T1
        34.5
        17.3%
        47%
        50 bp
        34.7
        1.2%
        16.4%
        47%
        49 bp
        34.7
        0.24%
        0.0
        34.5
        99.4%
        34.7
        16.7%
        MV411_1_293_R1
        27.8
        23.7
        125
        1.04
        0.66
        0.21%
        0.0
        27.8
        99.7%
        27.9
        0.19%
        0.0
        23.7
        100.0%
        23.7
        100%
        MV411_1_293_R1_T1
        27.8
        5.0%
        42%
        50 bp
        27.9
        1.2%
        4.8%
        43%
        49 bp
        27.9
        0.21%
        0.0
        27.8
        99.7%
        27.9
        3.5%
        MV411_1_319_R1
        28.6
        19.8
        165
        1.48
        1.88
        0.16%
        0.0
        28.6
        98.1%
        29.1
        0.15%
        0.0
        19.8
        100.0%
        19.8
        100%
        MV411_1_319_R1_T1
        28.6
        19.3%
        43%
        37 bp
        29.2
        1.4%
        19.0%
        43%
        36 bp
        29.1
        0.16%
        0.0
        28.6
        98.1%
        29.1
        15.3%
        MV411_1_320_R1
        26.0
        16.7
        160
        1.04
        0.82
        0.16%
        0.0
        26.0
        98.0%
        26.5
        0.14%
        0.0
        16.7
        100.0%
        16.7
        100%
        MV411_1_320_R1_T1
        26.0
        23.2%
        42%
        37 bp
        26.5
        1.5%
        22.8%
        42%
        36 bp
        26.5
        0.16%
        0.0
        26.0
        98.0%
        26.5
        18.2%
        MV411_1_324_R1
        20.6
        8.9
        145
        1.01
        0.33
        0.15%
        0.0
        20.6
        97.3%
        21.2
        0.13%
        0.0
        8.9
        100.0%
        8.9
        100%
        MV411_1_324_R1_T1
        20.6
        47.4%
        41%
        37 bp
        21.2
        1.6%
        46.6%
        41%
        36 bp
        21.2
        0.15%
        0.0
        20.6
        97.3%
        21.2
        41.1%
        MV411_1_357_R1
        52.7
        34.6
        155
        1.04
        1.08
        0.26%
        0.0
        52.7
        99.6%
        53.0
        0.21%
        0.0
        34.6
        100.0%
        34.6
        100%
        MV411_1_357_R1_T1
        52.7
        24.5%
        44%
        50 bp
        53.0
        1.1%
        23.9%
        44%
        49 bp
        53.0
        0.26%
        0.0
        52.7
        99.6%
        53.0
        21.2%
        MV411_1_359_R1
        55.0
        29.9
        145
        1.03
        0.87
        0.26%
        0.0
        55.0
        95.7%
        57.4
        0.20%
        0.0
        29.9
        100.0%
        29.9
        100%
        MV411_1_359_R1_T1
        55.0
        35.4%
        43%
        50 bp
        57.4
        3.2%
        34.9%
        43%
        48 bp
        57.4
        0.26%
        0.0
        55.0
        95.7%
        57.4
        33.3%
        MV411_1_430_R1
        37.0
        26.3
        130
        1.01
        0.47
        0.22%
        0.0
        37.0
        99.6%
        37.2
        0.17%
        0.0
        26.3
        100.0%
        26.3
        100%
        MV411_1_430_R1_T1
        37.0
        19.4%
        41%
        50 bp
        37.2
        1.2%
        18.8%
        42%
        49 bp
        37.2
        0.22%
        0.0
        37.0
        99.6%
        37.2
        16.4%
        MV411_1_433_R1
        35.3
        17.2
        90
        1.01
        0.33
        0.22%
        0.0
        35.3
        98.5%
        35.8
        0.16%
        0.0
        17.2
        100.0%
        17.2
        100%
        MV411_1_433_R1_T1
        35.3
        43.1%
        42%
        50 bp
        35.9
        1.2%
        41.9%
        42%
        49 bp
        35.8
        0.22%
        0.0
        35.3
        98.5%
        35.8
        39.5%
        MV411_1_565_R1
        40.5
        7.7
        135
        1.01
        0.24
        0.24%
        0.0
        40.5
        97.5%
        41.5
        0.17%
        0.0
        7.7
        100.0%
        7.7
        100%
        MV411_1_565_R1_T1
        40.5
        75.2%
        42%
        50 bp
        41.5
        1.4%
        73.8%
        42%
        49 bp
        41.5
        0.24%
        0.0
        40.5
        97.5%
        41.5
        71.6%
        MV411_1_570_R1
        68.0
        2.9
        125
        3.82
        1.97
        0.20%
        0.0
        68.0
        99.6%
        68.3
        0.15%
        0.0
        2.9
        100.0%
        2.9
        100%
        MV411_1_570_R1_T1
        68.0
        93.6%
        46%
        50 bp
        68.3
        1.1%
        92.6%
        46%
        49 bp
        68.3
        0.20%
        0.0
        68.0
        99.6%
        68.3
        91.7%
        MV411_1_577_R1
        47.2
        28.1
        125
        1.20
        1.31
        0.21%
        0.0
        47.2
        99.5%
        47.4
        0.15%
        0.0
        28.1
        100.0%
        28.1
        100%
        MV411_1_577_R1_T1
        47.2
        31.8%
        49%
        50 bp
        47.4
        1.1%
        31.0%
        49%
        49 bp
        47.4
        0.21%
        0.0
        47.2
        99.5%
        47.4
        28.1%
        MV411_1_581_R1
        50.2
        22.2
        140
        1.02
        0.78
        0.22%
        0.0
        50.2
        99.3%
        50.5
        0.16%
        0.0
        22.2
        100.0%
        22.2
        100%
        MV411_1_581_R1_T1
        50.2
        48.0%
        43%
        50 bp
        50.5
        1.1%
        47.4%
        43%
        49 bp
        50.5
        0.22%
        0.0
        50.2
        99.3%
        50.5
        44.1%
        MV411_1_582_R1
        43.0
        31.5
        145
        1.18
        1.38
        0.21%
        0.0
        43.0
        99.6%
        43.1
        0.16%
        0.0
        31.5
        100.0%
        31.5
        100%
        MV411_1_582_R1_T1
        43.0
        17.3%
        43%
        50 bp
        43.1
        1.1%
        17.0%
        44%
        49 bp
        43.1
        0.21%
        0.0
        43.0
        99.6%
        43.1
        14.0%
        MV411_1_583_R1
        35.7
        12.6
        135
        1.63
        1.37
        0.20%
        0.0
        35.7
        99.5%
        35.9
        0.15%
        0.0
        12.6
        100.0%
        12.6
        100%
        MV411_1_583_R1_T1
        35.7
        58.2%
        47%
        50 bp
        35.9
        1.1%
        57.5%
        47%
        49 bp
        35.9
        0.20%
        0.0
        35.7
        99.5%
        35.9
        54.9%
        MV411_1_587_R1
        44.7
        27.5
        140
        1.05
        0.93
        0.31%
        0.0
        44.7
        99.0%
        45.1
        0.17%
        0.0
        27.5
        100.0%
        27.5
        100%
        MV411_1_587_R1_T1
        44.7
        28.1%
        44%
        50 bp
        45.1
        1.0%
        27.9%
        44%
        49 bp
        45.1
        0.31%
        0.0
        44.7
        99.0%
        45.1
        25.8%
        MV411_1_590_R1
        33.8
        14.6
        130
        1.01
        0.56
        0.21%
        0.0
        33.8
        99.4%
        34.1
        0.15%
        0.0
        14.6
        100.0%
        14.6
        100%
        MV411_1_590_R1_T1
        33.8
        49.1%
        44%
        50 bp
        34.1
        1.1%
        48.8%
        44%
        49 bp
        34.1
        0.21%
        0.0
        33.8
        99.4%
        34.1
        44.9%
        MV411_1_601_R1
        39.5
        19.4
        125
        1.01
        0.45
        0.23%
        0.0
        39.5
        99.6%
        39.7
        0.16%
        0.0
        19.4
        100.0%
        19.4
        100%
        MV411_1_601_R1_T1
        39.5
        42.5%
        42%
        50 bp
        39.7
        1.2%
        41.4%
        42%
        49 bp
        39.7
        0.23%
        0.0
        39.5
        99.6%
        39.7
        38.8%
        MV411_1_639_R1
        51.8
        17.4
        165
        1.52
        1.14
        0.37%
        0.0
        51.8
        99.4%
        52.2
        0.22%
        0.0
        17.4
        100.0%
        17.4
        100%
        MV411_1_639_R1_T1
        51.8
        55.0%
        49%
        75 bp
        52.2
        1.3%
        52.7%
        49%
        73 bp
        52.2
        0.37%
        0.0
        51.8
        99.4%
        52.2
        60.8%
        MV411_1_640_R1
        88.2
        29.6
        160
        1.03
        1.00
        0.36%
        0.0
        88.2
        99.4%
        88.8
        0.20%
        0.0
        29.6
        100.0%
        29.6
        100%
        MV411_1_640_R1_T1
        88.2
        56.3%
        42%
        75 bp
        88.8
        1.4%
        54.2%
        42%
        73 bp
        88.8
        0.36%
        0.0
        88.2
        99.4%
        88.8
        59.5%
        MV411_1_650_R1
        46.9
        0.8
        110
        1.04
        0.20
        0.29%
        0.0
        46.9
        95.1%
        49.3
        0.22%
        0.0
        0.8
        100.0%
        0.8
        100%
        MV411_1_650_R1_T1
        46.9
        93.2%
        41%
        75 bp
        49.3
        1.8%
        92.6%
        41%
        73 bp
        49.3
        0.29%
        0.0
        46.9
        95.1%
        49.3
        95.2%
        MV411_1_70_R1
        28.1
        19.3
        145
        1.06
        0.96
        0.19%
        0.0
        28.1
        98.7%
        28.5
        0.17%
        0.0
        19.3
        100.0%
        19.3
        100%
        MV411_1_70_R1_T1
        28.1
        23.1%
        48%
        50 bp
        28.5
        1.7%
        22.5%
        48%
        49 bp
        28.5
        0.19%
        0.0
        28.1
        98.7%
        28.5
        21.3%
        MV411_1_71_R1
        16.7
        14.3
        150
        1.01
        0.64
        0.19%
        0.0
        16.7
        98.8%
        16.9
        0.17%
        0.0
        14.3
        100.0%
        14.3
        100%
        MV411_1_71_R1_T1
        16.7
        4.4%
        42%
        50 bp
        16.9
        1.2%
        4.3%
        42%
        49 bp
        16.9
        0.19%
        0.0
        16.7
        98.8%
        16.9
        2.6%
        MV411_1_72_R1
        17.4
        14.5
        150
        1.24
        1.54
        0.21%
        0.0
        17.4
        98.6%
        17.6
        0.17%
        0.0
        14.5
        100.0%
        14.5
        100%
        MV411_1_72_R1_T1
        17.4
        6.0%
        41%
        50 bp
        17.6
        1.1%
        5.9%
        41%
        49 bp
        17.6
        0.21%
        0.0
        17.4
        98.6%
        17.6
        3.8%
        MV411_1_767_R1
        50.2
        35.7
        245
        1.09
        1.83
        0.33%
        0.0
        50.2
        98.8%
        50.8
        0.21%
        0.0
        35.7
        100.0%
        35.7
        100%
        MV411_1_767_R1_T1
        50.2
        20.1%
        40%
        75 bp
        50.8
        0.9%
        19.4%
        40%
        74 bp
        50.8
        0.33%
        0.0
        50.2
        98.8%
        50.8
        20.9%
        MV411_1_771_R1
        52.4
        28.3
        235
        1.02
        1.79
        0.36%
        0.0
        52.4
        98.5%
        53.2
        0.22%
        0.0
        28.3
        100.0%
        28.3
        100%
        MV411_1_771_R1_T1
        52.4
        36.7%
        39%
        75 bp
        53.2
        1.0%
        35.5%
        39%
        74 bp
        53.2
        0.36%
        0.0
        52.4
        98.5%
        53.2
        38.5%
        MV411_1_773_R1
        53.1
        45.2
        235
        1.01
        0.61
        0.39%
        0.0
        53.1
        99.4%
        53.4
        0.26%
        0.0
        45.2
        100.0%
        45.2
        100%
        MV411_1_773_R1_T1
        53.1
        5.9%
        39%
        75 bp
        53.5
        1.0%
        5.6%
        39%
        74 bp
        53.4
        0.39%
        0.0
        53.1
        99.4%
        53.4
        6.0%
        MV411_1_89_R1
        20.7
        16.6
        115
        1.03
        0.52
        0.24%
        0.0
        20.7
        99.2%
        20.8
        0.17%
        0.0
        16.6
        100.0%
        16.6
        100%
        MV411_1_89_R1_T1
        20.7
        5.9%
        45%
        50 bp
        20.8
        1.4%
        5.9%
        45%
        49 bp
        20.8
        0.24%
        0.0
        20.7
        99.2%
        20.8
        3.1%
        MV411_2_112_R1
        21.8
        16.7
        160
        1.08
        1.42
        0.23%
        0.0
        21.8
        99.6%
        21.9
        0.18%
        0.0
        16.7
        100.0%
        16.7
        100%
        MV411_2_112_R1_T1
        21.8
        12.5%
        42%
        50 bp
        21.9
        1.1%
        12.2%
        42%
        49 bp
        21.9
        0.23%
        0.0
        21.8
        99.6%
        21.9
        9.9%
        MV411_2_116_R1
        32.2
        22.3
        160
        1.05
        1.23
        0.22%
        0.0
        32.2
        99.3%
        32.4
        0.18%
        0.0
        22.3
        100.0%
        22.3
        100%
        MV411_2_116_R1_T1
        32.2
        20.5%
        43%
        50 bp
        32.4
        1.1%
        19.9%
        43%
        49 bp
        32.4
        0.22%
        0.0
        32.2
        99.3%
        32.4
        17.5%
        MV411_2_129_R1
        27.9
        22.1
        110
        1.01
        0.45
        0.23%
        0.0
        27.9
        98.3%
        28.4
        0.18%
        0.0
        22.1
        100.0%
        22.1
        100%
        MV411_2_129_R1_T1
        27.9
        10.5%
        41%
        50 bp
        28.4
        1.2%
        10.3%
        41%
        49 bp
        28.4
        0.23%
        0.0
        27.9
        98.3%
        28.4
        7.6%
        MV411_2_301_R1
        22.9
        15.0
        140
        1.53
        1.51
        0.15%
        0.0
        22.9
        98.0%
        23.4
        0.14%
        0.0
        15.0
        100.0%
        15.0
        100%
        MV411_2_301_R1_T1
        22.9
        23.7%
        44%
        37 bp
        23.4
        1.5%
        23.3%
        44%
        36 bp
        23.4
        0.15%
        0.0
        22.9
        98.0%
        23.4
        20.1%
        MV411_2_325_R1
        30.7
        22.1
        175
        1.51
        1.36
        0.17%
        0.0
        30.7
        97.7%
        31.4
        0.15%
        0.0
        22.1
        100.0%
        22.1
        100%
        MV411_2_325_R1_T1
        30.7
        16.2%
        45%
        37 bp
        31.4
        1.5%
        15.8%
        46%
        36 bp
        31.4
        0.17%
        0.0
        30.7
        97.7%
        31.4
        12.4%
        MV411_2_358_R1
        51.3
        19.1
        145
        1.01
        0.59
        0.26%
        0.0
        51.3
        99.3%
        51.7
        0.19%
        0.0
        19.1
        100.0%
        19.1
        100%
        MV411_2_358_R1_T1
        51.3
        54.5%
        43%
        50 bp
        51.8
        1.1%
        53.8%
        43%
        49 bp
        51.7
        0.26%
        0.0
        51.3
        99.3%
        51.7
        51.5%
        MV411_2_427_R1
        40.3
        18.5
        140
        1.01
        0.60
        0.25%
        0.0
        40.3
        98.8%
        40.7
        0.19%
        0.0
        18.5
        100.0%
        18.5
        100%
        MV411_2_427_R1_T1
        40.3
        45.3%
        43%
        50 bp
        40.8
        1.2%
        44.7%
        43%
        49 bp
        40.7
        0.25%
        0.0
        40.3
        98.8%
        40.7
        42.2%
        MV411_2_429_R1
        33.8
        17.6
        165
        2.15
        1.70
        0.21%
        0.0
        33.8
        97.9%
        34.5
        0.16%
        0.0
        17.6
        100.0%
        17.6
        100%
        MV411_2_429_R1_T1
        33.8
        40.1%
        43%
        50 bp
        34.5
        1.2%
        39.0%
        43%
        49 bp
        34.5
        0.21%
        0.0
        33.8
        97.9%
        34.5
        38.2%
        MV411_2_431_R1
        32.3
        19.1
        135
        1.01
        0.45
        0.22%
        0.0
        32.3
        99.5%
        32.5
        0.17%
        0.0
        19.1
        100.0%
        19.1
        100%
        MV411_2_431_R1_T1
        32.3
        32.0%
        42%
        50 bp
        32.5
        1.2%
        31.1%
        42%
        49 bp
        32.5
        0.22%
        0.0
        32.3
        99.5%
        32.5
        28.8%
        MV411_2_435_R1
        44.7
        31.9
        185
        1.07
        1.25
        0.23%
        0.0
        44.7
        99.3%
        45.0
        0.19%
        0.0
        31.9
        100.0%
        31.9
        100%
        MV411_2_435_R1_T1
        44.7
        19.0%
        43%
        50 bp
        45.1
        1.2%
        18.3%
        43%
        49 bp
        45.0
        0.23%
        0.0
        44.7
        99.3%
        45.0
        16.7%
        MV411_2_584_R1
        40.3
        20.7
        140
        1.18
        1.14
        0.21%
        0.0
        40.3
        99.5%
        40.5
        0.16%
        0.0
        20.7
        100.0%
        20.7
        100%
        MV411_2_584_R1_T1
        40.3
        40.7%
        46%
        50 bp
        40.5
        1.1%
        40.1%
        46%
        49 bp
        40.5
        0.21%
        0.0
        40.3
        99.5%
        40.5
        37.4%
        MV411_2_604_R1
        26.1
        16.1
        135
        1.01
        0.47
        0.26%
        0.0
        26.1
        98.8%
        26.5
        0.18%
        0.0
        16.1
        100.0%
        16.1
        100%
        MV411_2_604_R1_T1
        26.1
        28.6%
        42%
        50 bp
        26.5
        1.2%
        27.8%
        42%
        49 bp
        26.5
        0.26%
        0.0
        26.1
        98.8%
        26.5
        25.7%
        MV411_2_614_R1
        69.4
        21.6
        175
        1.01
        0.63
        0.21%
        0.0
        69.4
        99.4%
        69.8
        0.16%
        0.0
        21.6
        100.0%
        21.6
        100%
        MV411_2_614_R1_T1
        69.4
        66.5%
        42%
        50 bp
        69.8
        1.2%
        66.0%
        42%
        49 bp
        69.8
        0.21%
        0.0
        69.4
        99.4%
        69.8
        58.8%
        MV411_2_768_R1
        67.7
        50.9
        265
        1.03
        1.40
        0.34%
        0.0
        67.7
        99.1%
        68.3
        0.22%
        0.0
        50.9
        100.0%
        50.9
        100%
        MV411_2_768_R1_T1
        67.7
        16.0%
        40%
        75 bp
        68.3
        0.9%
        15.5%
        40%
        74 bp
        68.3
        0.34%
        0.0
        67.7
        99.1%
        68.3
        16.6%
        MV411_2_770_R1
        51.7
        41.3
        245
        1.01
        0.70
        0.35%
        0.0
        51.7
        99.1%
        52.1
        0.22%
        0.0
        41.3
        100.0%
        41.3
        100%
        MV411_2_770_R1_T1
        51.7
        11.2%
        39%
        75 bp
        52.2
        0.9%
        10.8%
        39%
        74 bp
        52.1
        0.35%
        0.0
        51.7
        99.1%
        52.1
        11.3%
        MV411_2_772_R1
        46.0
        21.5
        235
        1.03
        2.15
        0.36%
        0.0
        46.0
        97.0%
        47.4
        0.22%
        0.0
        21.5
        100.0%
        21.5
        100%
        MV411_2_772_R1_T1
        46.0
        44.2%
        39%
        75 bp
        47.5
        1.0%
        42.7%
        39%
        74 bp
        47.4
        0.36%
        0.0
        46.0
        97.0%
        47.4
        46.0%
        MV411_2_774_R1
        41.7
        14.3
        215
        1.06
        2.80
        0.39%
        0.0
        41.7
        94.1%
        44.3
        0.22%
        0.0
        14.3
        100.0%
        14.3
        100%
        MV411_2_774_R1_T1
        41.7
        55.7%
        40%
        75 bp
        44.5
        1.4%
        54.0%
        40%
        74 bp
        44.3
        0.39%
        0.0
        41.7
        94.1%
        44.3
        58.2%
        MV411_2_88_R1
        17.8
        15.4
        135
        1.12
        1.03
        0.19%
        0.0
        17.8
        99.5%
        17.9
        0.17%
        0.0
        15.4
        100.0%
        15.4
        100%
        MV411_2_88_R1_T1
        17.8
        3.1%
        48%
        50 bp
        17.9
        1.5%
        3.1%
        48%
        49 bp
        17.9
        0.19%
        0.0
        17.8
        99.5%
        17.9
        1.7%
        MV411_3_136_R1
        40.7
        31.9
        190
        1.02
        0.91
        0.24%
        0.0
        40.7
        99.6%
        40.9
        0.20%
        0.0
        31.9
        100.0%
        31.9
        100%
        MV411_3_136_R1_T1
        40.7
        12.0%
        41%
        50 bp
        40.9
        1.3%
        11.3%
        41%
        49 bp
        40.9
        0.24%
        0.0
        40.7
        99.6%
        40.9
        9.2%
        MV411_3_299_R1
        44.4
        33.1
        170
        1.49
        1.99
        0.18%
        0.0
        44.4
        97.2%
        45.7
        0.14%
        0.0
        33.1
        100.0%
        33.1
        100%
        MV411_3_299_R1_T1
        44.4
        14.3%
        40%
        37 bp
        45.7
        1.5%
        14.0%
        41%
        36 bp
        45.7
        0.18%
        0.0
        44.4
        97.2%
        45.7
        9.6%
        MV411_3_300_R1
        24.4
        17.5
        155
        1.15
        1.39
        0.16%
        0.0
        24.4
        95.9%
        25.5
        0.15%
        0.0
        17.5
        100.0%
        17.5
        100%
        MV411_3_300_R1_T1
        24.4
        15.7%
        43%
        37 bp
        25.6
        1.8%
        15.3%
        44%
        36 bp
        25.5
        0.16%
        0.0
        24.4
        95.9%
        25.5
        11.4%
        MV411_3_425_R1
        53.1
        5.7
        145
        1.17
        1.12
        0.22%
        0.0
        53.1
        97.5%
        54.5
        0.16%
        0.0
        5.7
        100.0%
        5.7
        100%
        MV411_3_425_R1_T1
        53.1
        85.1%
        43%
        50 bp
        54.5
        1.3%
        83.8%
        44%
        49 bp
        54.5
        0.22%
        0.0
        53.1
        97.5%
        54.5
        81.3%
        MV411_3_426_R1
        46.5
        29.8
        150
        1.11
        1.18
        0.26%
        0.0
        46.5
        99.4%
        46.8
        0.20%
        0.0
        29.8
        100.0%
        29.8
        100%
        MV411_3_426_R1_T1
        46.5
        26.3%
        44%
        50 bp
        46.8
        1.1%
        25.9%
        44%
        49 bp
        46.8
        0.26%
        0.0
        46.5
        99.4%
        46.8
        23.8%
        MV411_3_428_R1
        28.1
        11.8
        145
        2.85
        1.56
        0.23%
        0.0
        28.1
        99.5%
        28.3
        0.18%
        0.0
        11.8
        100.0%
        11.8
        100%
        MV411_3_428_R1_T1
        28.1
        50.8%
        44%
        50 bp
        28.3
        1.1%
        50.1%
        45%
        49 bp
        28.3
        0.23%
        0.0
        28.1
        99.5%
        28.3
        49.8%
        MV411_3_568_R1
        45.8
        5.8
        125
        1.02
        0.43
        0.22%
        0.0
        45.8
        99.3%
        46.1
        0.15%
        0.0
        5.8
        100.0%
        5.8
        100%
        MV411_3_568_R1_T1
        45.8
        83.6%
        41%
        50 bp
        46.1
        1.1%
        82.5%
        42%
        49 bp
        46.1
        0.22%
        0.0
        45.8
        99.3%
        46.1
        79.1%
        MV411_3_575_R1
        55.9
        13.0
        130
        1.01
        0.36
        0.21%
        0.0
        55.9
        99.5%
        56.2
        0.15%
        0.0
        13.0
        100.0%
        13.0
        100%
        MV411_3_575_R1_T1
        55.9
        71.6%
        41%
        50 bp
        56.2
        1.1%
        70.6%
        41%
        49 bp
        56.2
        0.21%
        0.0
        55.9
        99.5%
        56.2
        67.2%
        MV411_3_585_R1
        38.0
        13.6
        135
        1.07
        0.99
        0.21%
        0.0
        38.0
        99.4%
        38.2
        0.15%
        0.0
        13.6
        100.0%
        13.6
        100%
        MV411_3_585_R1_T1
        38.0
        57.1%
        45%
        50 bp
        38.2
        1.1%
        56.3%
        45%
        49 bp
        38.2
        0.21%
        0.0
        38.0
        99.4%
        38.2
        53.2%
        MV411_3_607_R1
        41.9
        16.5
        125
        1.03
        0.93
        0.22%
        0.0
        41.9
        99.5%
        42.1
        0.16%
        0.0
        16.5
        100.0%
        16.5
        100%
        MV411_3_607_R1_T1
        41.9
        53.5%
        41%
        50 bp
        42.1
        1.2%
        52.4%
        41%
        49 bp
        42.1
        0.22%
        0.0
        41.9
        99.5%
        42.1
        49.9%
        MV411_3_623_R1
        28.7
        20.0
        195
        1.00
        0.29
        0.20%
        0.0
        28.7
        99.4%
        28.9
        0.16%
        0.0
        20.0
        100.0%
        20.0
        100%
        MV411_3_623_R1_T1
        28.7
        20.5%
        42%
        50 bp
        28.9
        1.1%
        20.2%
        42%
        49 bp
        28.9
        0.20%
        0.0
        28.7
        99.4%
        28.9
        17.5%
        MV411_4_321_R1
        29.7
        22.2
        140
        1.02
        0.92
        0.15%
        0.0
        29.7
        98.2%
        30.2
        0.14%
        0.0
        22.2
        100.0%
        22.2
        100%
        MV411_4_321_R1_T1
        29.7
        12.3%
        42%
        37 bp
        30.2
        1.5%
        12.1%
        42%
        36 bp
        30.2
        0.15%
        0.0
        29.7
        98.2%
        30.2
        7.3%
        MV411_4_424_R1
        61.1
        19.3
        140
        1.12
        1.26
        0.25%
        0.0
        61.1
        98.5%
        62.0
        0.18%
        0.0
        19.3
        100.0%
        19.3
        100%
        MV411_4_424_R1_T1
        61.1
        61.4%
        43%
        50 bp
        62.1
        1.1%
        60.7%
        43%
        49 bp
        62.0
        0.25%
        0.0
        61.1
        98.5%
        62.0
        58.6%
        MV411_4_569_R1
        39.2
        14.1
        140
        1.03
        0.75
        0.23%
        0.0
        39.2
        98.2%
        39.9
        0.16%
        0.0
        14.1
        100.0%
        14.1
        100%
        MV411_4_569_R1_T1
        39.2
        56.9%
        41%
        50 bp
        39.9
        1.3%
        55.5%
        41%
        49 bp
        39.9
        0.23%
        0.0
        39.2
        98.2%
        39.9
        53.7%
        MV411_4_627_R1
        81.5
        36.5
        155
        1.01
        0.57
        0.32%
        0.0
        81.5
        99.3%
        82.0
        0.19%
        0.0
        36.5
        100.0%
        36.5
        100%
        MV411_4_627_R1_T1
        81.5
        46.5%
        39%
        75 bp
        82.0
        1.1%
        45.1%
        39%
        73 bp
        82.0
        0.32%
        0.0
        81.5
        99.3%
        82.0
        48.0%
        MV411_4_717_R1
        72.6
        5.2
        130
        1.82
        1.16
        0.77%
        0.0
        72.6
        95.3%
        76.1
        0.27%
        0.0
        5.2
        100.0%
        5.2
        100%
        MV411_4_717_R1_T1
        72.6
        78.7%
        41%
        75 bp
        76.2
        2.6%
        77.0%
        41%
        72 bp
        76.1
        0.77%
        0.0
        72.6
        95.3%
        76.1
        88.0%
        MV411_5_589_R1
        24.1
        12.2
        120
        1.02
        0.74
        0.22%
        0.0
        24.1
        99.4%
        24.3
        0.16%
        0.0
        12.2
        100.0%
        12.2
        100%
        MV411_5_589_R1_T1
        24.1
        40.6%
        44%
        50 bp
        24.3
        1.1%
        40.4%
        44%
        49 bp
        24.3
        0.22%
        0.0
        24.1
        99.4%
        24.3
        37.0%
        MV411_6_763_R1
        48.5
        36.2
        250
        1.23
        1.97
        0.31%
        0.0
        48.5
        99.2%
        48.9
        0.22%
        0.0
        36.2
        100.0%
        36.2
        100%
        MV411_6_763_R1_T1
        48.5
        16.8%
        42%
        75 bp
        48.9
        0.9%
        16.3%
        42%
        74 bp
        48.9
        0.31%
        0.0
        48.5
        99.2%
        48.9
        17.8%
        MV411_7_714_R1
        140.7
        49.8
        120
        1.01
        0.73
        0.80%
        0.0
        140.7
        98.7%
        142.6
        0.42%
        0.0
        49.8
        100.0%
        49.8
        100%
        MV411_7_714_R1_T1
        140.7
        46.4%
        41%
        75 bp
        142.6
        1.4%
        44.0%
        41%
        73 bp
        142.6
        0.80%
        0.0
        140.7
        98.7%
        142.6
        57.3%
        MV411_7_764_R1
        48.4
        35.0
        265
        1.31
        2.11
        0.31%
        0.0
        48.4
        99.0%
        48.9
        0.21%
        0.0
        35.0
        100.0%
        35.0
        100%
        MV411_7_764_R1_T1
        48.4
        19.2%
        42%
        75 bp
        48.9
        0.9%
        18.6%
        42%
        74 bp
        48.9
        0.31%
        0.0
        48.4
        99.0%
        48.9
        20.2%
        MV411_8_715_R1
        80.6
        20.2
        165
        1.06
        0.89
        0.81%
        0.0
        80.6
        98.1%
        82.2
        0.37%
        0.0
        20.2
        100.0%
        20.2
        100%
        MV411_8_715_R1_T1
        80.6
        56.6%
        42%
        75 bp
        82.2
        1.6%
        54.1%
        42%
        73 bp
        82.2
        0.81%
        0.0
        80.6
        98.1%
        82.2
        68.3%
        MV411_8_765_R1
        49.0
        35.8
        255
        1.20
        1.74
        0.33%
        0.0
        49.0
        97.8%
        50.1
        0.23%
        0.0
        35.8
        100.0%
        35.8
        100%
        MV411_8_765_R1_T1
        49.0
        18.6%
        42%
        75 bp
        50.3
        1.3%
        17.8%
        42%
        74 bp
        50.1
        0.33%
        0.0
        49.0
        97.8%
        50.1
        19.3%
        MV411_9_716_R1
        68.9
        6.4
        135
        1.01
        0.20
        0.76%
        0.0
        68.9
        95.0%
        72.5
        0.27%
        0.0
        6.4
        100.0%
        6.4
        100%
        MV411_9_716_R1_T1
        68.9
        76.0%
        40%
        75 bp
        72.5
        1.5%
        73.9%
        40%
        73 bp
        72.5
        0.76%
        0.0
        68.9
        95.0%
        72.5
        85.5%
        MV411_9_718_R1
        64.5
        22.5
        120
        1.01
        0.65
        0.78%
        0.0
        64.5
        98.6%
        65.4
        0.41%
        0.0
        22.5
        100.0%
        22.5
        100%
        MV411_9_718_R1_T1
        64.5
        46.8%
        41%
        75 bp
        65.4
        1.4%
        44.4%
        41%
        73 bp
        65.4
        0.78%
        0.0
        64.5
        98.6%
        65.4
        57.7%
        MV411_DMSO_8h_1_690_R1
        54.0
        4.1
        185
        1.98
        1.23
        0.38%
        0.0
        54.0
        98.0%
        55.1
        0.22%
        0.0
        4.1
        100.0%
        4.1
        100%
        MV411_DMSO_8h_1_690_R1_T1
        54.0
        82.2%
        48%
        75 bp
        55.1
        1.3%
        80.4%
        48%
        73 bp
        55.1
        0.38%
        0.0
        54.0
        98.0%
        55.1
        89.1%
        MV411_DMSO_8h_2_702_R1
        46.8
        34.5
        195
        1.16
        1.14
        0.31%
        0.0
        46.8
        98.8%
        47.4
        0.24%
        0.0
        34.5
        100.0%
        34.5
        100%
        MV411_DMSO_8h_2_702_R1_T1
        46.8
        18.7%
        45%
        75 bp
        47.4
        1.4%
        18.1%
        45%
        73 bp
        47.4
        0.31%
        0.0
        46.8
        98.8%
        47.4
        19.6%
        NB4_1_193_R1
        20.4
        17.8
        175
        1.08
        1.12
        0.18%
        0.0
        20.4
        99.8%
        20.4
        0.16%
        0.0
        17.8
        100.0%
        17.8
        100%
        NB4_1_193_R1_T1
        20.4
        2.7%
        47%
        50 bp
        20.4
        1.0%
        2.7%
        47%
        49 bp
        20.4
        0.18%
        0.0
        20.4
        99.8%
        20.4
        1.3%
        NB4_2_203_R1
        54.2
        38.4
        150
        1.14
        1.14
        0.18%
        0.0
        54.2
        99.8%
        54.3
        0.16%
        0.0
        38.4
        100.0%
        38.4
        100%
        NB4_2_203_R1_T1
        54.2
        20.8%
        50%
        50 bp
        54.3
        1.1%
        20.5%
        50%
        49 bp
        54.3
        0.18%
        0.0
        54.2
        99.8%
        54.3
        18.8%
        NOMO1_2_92_R1
        21.9
        19.0
        125
        1.07
        0.98
        0.19%
        0.0
        21.9
        99.5%
        22.0
        0.18%
        0.0
        19.0
        100.0%
        19.0
        100%
        NOMO1_2_92_R1_T1
        21.9
        3.5%
        47%
        50 bp
        22.0
        1.6%
        3.5%
        48%
        49 bp
        22.0
        0.19%
        0.0
        21.9
        99.5%
        22.0
        2.4%
        NOMO1_3_186_R1
        22.3
        18.9
        170
        1.45
        1.19
        0.18%
        0.0
        22.3
        99.9%
        22.4
        0.16%
        0.0
        18.9
        100.0%
        18.9
        100%
        NOMO1_3_186_R1_T1
        22.3
        7.1%
        50%
        50 bp
        22.4
        1.0%
        7.0%
        50%
        50 bp
        22.4
        0.18%
        0.0
        22.3
        99.9%
        22.4
        6.3%
        OCIAML2_1_168_R1
        80.3
        55.6
        145
        1.01
        0.79
        0.27%
        0.0
        80.3
        99.3%
        80.9
        0.21%
        0.0
        55.6
        100.0%
        55.6
        100%
        OCIAML2_1_168_R1_T1
        80.3
        21.5%
        40%
        50 bp
        80.9
        1.2%
        20.6%
        40%
        49 bp
        80.9
        0.27%
        0.0
        80.3
        99.3%
        80.9
        19.1%
        OCIAML2_1_45_R1
        47.5
        41.9
        0
        1.02
        0.63
        0.34%
        0.0
        47.5
        99.9%
        47.6
        0.18%
        0.0
        41.9
        100.0%
        41.9
        100%
        OCIAML2_1_45_R1_T1
        47.5
        7.0%
        42%
        150 bp
        47.6
        15.3%
        6.5%
        42%
        127 bp
        47.6
        0.34%
        0.0
        47.5
        99.9%
        47.6
        5.2%
        OCIAML2_2_41_R1
        32.1
        25.7
        -120
        1.02
        0.06
        0.42%
        0.0
        32.1
        99.6%
        32.2
        0.20%
        0.0
        25.7
        100.0%
        25.7
        100%
        OCIAML2_2_41_R1_T1
        32.1
        13.3%
        43%
        150 bp
        32.2
        15.8%
        12.8%
        41%
        126 bp
        32.2
        0.42%
        0.0
        32.1
        99.6%
        32.2
        12.4%
        OCIAML2_2_50_R1
        35.0
        30.1
        70
        1.11
        0.96
        0.18%
        0.0
        35.0
        99.0%
        35.3
        0.16%
        0.0
        30.1
        100.0%
        30.1
        100%
        OCIAML2_2_50_R1_T1
        35.0
        3.6%
        46%
        50 bp
        35.3
        2.3%
        3.6%
        47%
        49 bp
        35.3
        0.18%
        0.0
        35.0
        99.0%
        35.3
        2.2%
        OCIAML2_3_67_R1
        32.7
        28.7
        120
        1.05
        0.91
        0.19%
        0.0
        32.7
        99.5%
        32.9
        0.18%
        0.0
        28.7
        100.0%
        28.7
        100%
        OCIAML2_3_67_R1_T1
        32.7
        2.8%
        45%
        50 bp
        32.9
        1.6%
        2.8%
        45%
        49 bp
        32.9
        0.19%
        0.0
        32.7
        99.5%
        32.9
        1.4%
        OCIAML3_1_195_R1
        22.6
        19.5
        190
        1.09
        1.21
        0.19%
        0.0
        22.6
        99.8%
        22.7
        0.17%
        0.0
        19.5
        100.0%
        19.5
        100%
        OCIAML3_1_195_R1_T1
        22.6
        4.9%
        44%
        50 bp
        22.7
        1.1%
        4.8%
        44%
        49 bp
        22.7
        0.19%
        0.0
        22.6
        99.8%
        22.7
        3.6%
        OCIAML3_2_205_R1
        14.2
        11.9
        155
        1.31
        1.22
        0.15%
        0.0
        14.2
        99.7%
        14.3
        0.14%
        0.0
        11.9
        100.0%
        11.9
        100%
        OCIAML3_2_205_R1_T1
        14.2
        7.2%
        49%
        50 bp
        14.3
        1.2%
        6.9%
        49%
        49 bp
        14.3
        0.15%
        0.0
        14.2
        99.7%
        14.3
        5.9%
        P31FUJ_4_776_R1
        57.9
        50.4
        275
        1.02
        1.06
        0.39%
        0.0
        57.9
        99.6%
        58.2
        0.27%
        0.0
        50.4
        100.0%
        50.4
        100%
        P31FUJ_4_776_R1_T1
        57.9
        4.6%
        40%
        75 bp
        58.2
        0.9%
        4.4%
        40%
        74 bp
        58.2
        0.39%
        0.0
        57.9
        99.6%
        58.2
        5.0%
        PLB985_1_597_R1
        31.9
        27.0
        165
        1.26
        1.11
        0.18%
        0.0
        31.9
        99.8%
        31.9
        0.16%
        0.0
        27.0
        100.0%
        27.0
        100%
        PLB985_1_597_R1_T1
        31.9
        6.3%
        49%
        50 bp
        31.9
        1.1%
        6.0%
        49%
        49 bp
        31.9
        0.18%
        0.0
        31.9
        99.8%
        31.9
        5.0%
        PLB985_2_598_R1
        33.0
        27.6
        165
        1.24
        1.11
        0.18%
        0.0
        33.0
        99.8%
        33.0
        0.16%
        0.0
        27.6
        100.0%
        27.6
        100%
        PLB985_2_598_R1_T1
        33.0
        7.5%
        49%
        50 bp
        33.0
        1.1%
        7.1%
        49%
        49 bp
        33.0
        0.18%
        0.0
        33.0
        99.8%
        33.0
        6.1%
        SKNO1_1_197_R1
        22.5
        19.0
        165
        1.30
        1.14
        0.18%
        0.0
        22.5
        99.8%
        22.5
        0.16%
        0.0
        19.0
        100.0%
        19.0
        100%
        SKNO1_1_197_R1_T1
        22.5
        7.6%
        50%
        50 bp
        22.5
        1.0%
        7.4%
        50%
        50 bp
        22.5
        0.18%
        0.0
        22.5
        99.8%
        22.5
        6.5%
        SKNO1_1_230_R1
        16.4
        13.6
        205
        1.23
        1.41
        0.21%
        0.0
        16.4
        98.6%
        16.7
        0.18%
        0.0
        13.6
        100.0%
        13.6
        100%
        SKNO1_1_230_R1_T1
        16.4
        6.5%
        44%
        50 bp
        16.7
        1.1%
        6.4%
        44%
        49 bp
        16.7
        0.21%
        0.0
        16.4
        98.6%
        16.7
        4.5%
        SKNO1_1_231_R1
        12.8
        9.1
        170
        1.13
        1.43
        0.22%
        0.0
        12.8
        97.5%
        13.2
        0.17%
        0.0
        9.1
        100.0%
        9.1
        100%
        SKNO1_1_231_R1_T1
        12.8
        19.3%
        42%
        50 bp
        13.2
        1.1%
        19.2%
        43%
        49 bp
        13.2
        0.22%
        0.0
        12.8
        97.5%
        13.2
        17.5%
        SKNO1_1_232_R1
        17.5
        13.0
        160
        1.05
        1.02
        0.22%
        0.0
        17.5
        98.2%
        17.8
        0.18%
        0.0
        13.0
        100.0%
        13.0
        100%
        SKNO1_1_232_R1_T1
        17.5
        15.5%
        42%
        50 bp
        17.8
        1.1%
        15.3%
        43%
        49 bp
        17.8
        0.22%
        0.0
        17.5
        98.2%
        17.8
        13.1%
        SKNO1_1_233_R1
        18.5
        15.1
        185
        1.04
        0.96
        0.22%
        0.0
        18.5
        98.3%
        18.9
        0.18%
        0.0
        15.1
        100.0%
        15.1
        100%
        SKNO1_1_233_R1_T1
        18.5
        7.8%
        43%
        50 bp
        18.9
        1.1%
        7.7%
        43%
        49 bp
        18.9
        0.22%
        0.0
        18.5
        98.3%
        18.9
        5.5%
        SKNO1_1_305_R1
        27.5
        15.5
        140
        1.14
        1.36
        0.15%
        0.0
        27.5
        98.2%
        28.0
        0.14%
        0.0
        15.5
        100.0%
        15.5
        100%
        SKNO1_1_305_R1_T1
        27.5
        33.7%
        42%
        37 bp
        28.0
        1.5%
        33.2%
        42%
        36 bp
        28.0
        0.15%
        0.0
        27.5
        98.2%
        28.0
        29.0%
        SKNO1_1_308_R1
        26.1
        17.6
        165
        1.08
        1.28
        0.16%
        0.0
        26.1
        97.2%
        26.8
        0.14%
        0.0
        17.6
        100.0%
        17.6
        100%
        SKNO1_1_308_R1_T1
        26.1
        20.2%
        43%
        37 bp
        26.9
        1.5%
        19.8%
        43%
        36 bp
        26.8
        0.16%
        0.0
        26.1
        97.2%
        26.8
        15.4%
        SKNO1_1_633_R1
        117.9
        68.8
        125
        1.05
        1.17
        0.46%
        0.0
        117.9
        99.2%
        118.8
        0.29%
        0.0
        68.8
        100.0%
        68.8
        100%
        SKNO1_1_633_R1_T1
        117.9
        29.3%
        42%
        75 bp
        118.8
        1.2%
        27.6%
        42%
        73 bp
        118.8
        0.46%
        0.0
        117.9
        99.2%
        118.8
        33.8%
        SKNO1_1_635_R1
        100.3
        57.3
        160
        1.13
        1.20
        0.46%
        0.0
        100.3
        99.2%
        101.0
        0.30%
        0.0
        57.3
        100.0%
        57.3
        100%
        SKNO1_1_635_R1_T1
        100.3
        30.3%
        43%
        75 bp
        101.0
        1.2%
        28.5%
        43%
        73 bp
        101.0
        0.46%
        0.0
        100.3
        99.2%
        101.0
        35.2%
        SKNO1_1_638_R1
        143.3
        36.5
        160
        1.14
        1.12
        0.36%
        0.0
        143.3
        99.4%
        144.1
        0.21%
        0.0
        36.5
        100.0%
        36.5
        100%
        SKNO1_1_638_R1_T1
        143.3
        64.0%
        46%
        75 bp
        144.1
        1.4%
        61.8%
        46%
        73 bp
        144.1
        0.36%
        0.0
        143.3
        99.4%
        144.1
        69.3%
        SKNO1_2_216_R1
        28.7
        25.1
        175
        1.08
        1.19
        0.19%
        0.0
        28.7
        99.8%
        28.8
        0.17%
        0.0
        25.1
        100.0%
        25.1
        100%
        SKNO1_2_216_R1_T1
        28.7
        3.4%
        44%
        50 bp
        28.8
        1.1%
        3.2%
        44%
        49 bp
        28.8
        0.19%
        0.0
        28.7
        99.8%
        28.8
        1.6%
        SKNO1_2_631_R1
        80.8
        29.5
        145
        1.34
        1.28
        0.43%
        0.0
        80.8
        98.9%
        81.7
        0.25%
        0.0
        29.5
        100.0%
        29.5
        100%
        SKNO1_2_631_R1_T1
        80.8
        50.5%
        42%
        75 bp
        81.7
        1.2%
        47.8%
        42%
        73 bp
        81.7
        0.43%
        0.0
        80.8
        98.9%
        81.7
        56.8%
        SKNO1_2_636_R1
        84.3
        22.4
        105
        1.06
        0.99
        0.44%
        0.0
        84.3
        98.8%
        85.4
        0.23%
        0.0
        22.4
        100.0%
        22.4
        100%
        SKNO1_2_636_R1_T1
        84.3
        60.6%
        43%
        75 bp
        85.4
        1.4%
        57.9%
        43%
        73 bp
        85.4
        0.44%
        0.0
        84.3
        98.8%
        85.4
        67.2%
        SKNO1_2_637_R1
        55.1
        31.0
        155
        1.04
        1.08
        0.36%
        0.0
        55.1
        99.6%
        55.4
        0.23%
        0.0
        31.0
        100.0%
        31.0
        100%
        SKNO1_2_637_R1_T1
        55.1
        33.5%
        42%
        75 bp
        55.4
        1.2%
        31.9%
        42%
        73 bp
        55.4
        0.36%
        0.0
        55.1
        99.6%
        55.4
        35.8%
        TF1_1_194_R1
        39.0
        33.6
        185
        1.19
        1.14
        0.17%
        0.0
        39.0
        99.8%
        39.1
        0.16%
        0.0
        33.6
        100.0%
        33.6
        100%
        TF1_1_194_R1_T1
        39.0
        5.9%
        48%
        50 bp
        39.1
        1.0%
        5.8%
        48%
        50 bp
        39.1
        0.17%
        0.0
        39.0
        99.8%
        39.1
        5.0%
        TF1_2_204_R1
        15.2
        12.8
        160
        1.33
        1.15
        0.15%
        0.0
        15.2
        99.8%
        15.2
        0.14%
        0.0
        12.8
        100.0%
        12.8
        100%
        TF1_2_204_R1_T1
        15.2
        7.7%
        50%
        50 bp
        15.2
        1.1%
        7.4%
        50%
        49 bp
        15.2
        0.15%
        0.0
        15.2
        99.8%
        15.2
        6.8%
        U937_1_73_R1
        38.3
        33.1
        145
        1.12
        1.00
        0.18%
        0.0
        38.3
        98.9%
        38.8
        0.17%
        0.0
        33.1
        100.0%
        33.1
        100%
        U937_1_73_R1_T1
        38.3
        5.4%
        49%
        50 bp
        38.8
        1.5%
        5.2%
        50%
        49 bp
        38.8
        0.18%
        0.0
        38.3
        98.9%
        38.8
        4.2%
        U937_2_90_R1
        27.4
        23.5
        135
        1.25
        1.03
        0.19%
        0.0
        27.4
        99.6%
        27.5
        0.17%
        0.0
        23.5
        100.0%
        23.5
        100%
        U937_2_90_R1_T1
        27.4
        5.0%
        50%
        50 bp
        27.5
        1.4%
        4.9%
        50%
        49 bp
        27.5
        0.19%
        0.0
        27.4
        99.6%
        27.5
        4.0%
        U937_3_213_R1
        23.1
        20.2
        210
        1.16
        1.39
        0.19%
        0.0
        23.1
        99.8%
        23.2
        0.17%
        0.0
        20.2
        100.0%
        20.2
        100%
        U937_3_213_R1_T1
        23.1
        3.3%
        46%
        50 bp
        23.2
        1.1%
        3.2%
        46%
        49 bp
        23.2
        0.19%
        0.0
        23.1
        99.8%
        23.2
        2.0%
        UCSDAML1_2_188_R1
        15.6
        13.2
        140
        1.40
        1.14
        0.18%
        0.0
        15.6
        99.8%
        15.7
        0.16%
        0.0
        13.2
        100.0%
        13.2
        100%
        UCSDAML1_2_188_R1_T1
        15.6
        6.8%
        51%
        50 bp
        15.7
        1.0%
        6.8%
        51%
        50 bp
        15.7
        0.18%
        0.0
        15.6
        99.8%
        15.7
        5.8%
        UT7_3_187_R1
        20.9
        17.7
        150
        1.09
        1.11
        0.20%
        0.0
        20.9
        99.7%
        21.0
        0.17%
        0.0
        17.7
        100.0%
        17.7
        100%
        UT7_3_187_R1_T1
        20.9
        5.5%
        46%
        50 bp
        21.0
        1.0%
        5.4%
        46%
        49 bp
        21.0
        0.20%
        0.0
        20.9
        99.7%
        21.0
        3.6%
        UT7_4_214_R1
        23.5
        20.6
        195
        1.10
        1.27
        0.19%
        0.0
        23.5
        99.8%
        23.6
        0.17%
        0.0
        20.6
        100.0%
        20.6
        100%
        UT7_4_214_R1_T1
        23.5
        3.0%
        44%
        50 bp
        23.6
        1.1%
        2.9%
        44%
        49 bp
        23.6
        0.19%
        0.0
        23.5
        99.8%
        23.6
        1.4%

        FastQC (library; raw)

        FastQC (library; raw) This section of the report shows FastQC results before adapter trimming.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (50bp , 75bp , 100bp , 37bp , 150bp). See the General Statistics Table.

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        loading..

        cutadapt (library; trimmed)

        cutadapt (library; trimmed) This section of the report shows the length of trimmed reads by cutadapt.

        This plot shows the number of reads with certain lengths of adapter trimmed. Obs/Exp shows the raw counts divided by the number expected due to sequencing errors. A defined peak may be related to adapter length. See the cutadapt documentation for more information on how these numbers are generated.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        FastQC (library; trimmed)

        FastQC (library; trimmed) This section of the report shows FastQC results after adapter trimming.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        No samples found with any adapter contamination > 0.1%

        SAMTools (library)

        Samtools This section of the report shows SAMTools results for individual libraries.

        Percent Mapped

        Alignment metrics from samtools stats; mapped vs. unmapped reads.

        For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

        Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Alignment metrics

        This module parses the output from samtools stats. All numbers in millions.

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        Samtools Flagstat

        This module parses the output from samtools flagstat. All numbers in millions.

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        XY counts

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Mapped reads per contig

        The samtools idxstats tool counts the number of mapped reads per chromosome / contig. Chromosomes with < 0.1% of the total aligned reads are omitted from this plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        SAMTools (merged library; unfiltered)

        Samtools This section of the report shows SAMTools results after merging libraries and before filtering.

        Percent Mapped

        Alignment metrics from samtools stats; mapped vs. unmapped reads.

        For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

        Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Alignment metrics

        This module parses the output from samtools stats. All numbers in millions.

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        Samtools Flagstat

        This module parses the output from samtools flagstat. All numbers in millions.

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        XY counts

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Mapped reads per contig

        The samtools idxstats tool counts the number of mapped reads per chromosome / contig. Chromosomes with < 0.1% of the total aligned reads are omitted from this plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        SAMTools (merged library; filtered)

        Samtools This section of the report shows SAMTools results after merging libraries and after filtering.

        Percent Mapped

        Alignment metrics from samtools stats; mapped vs. unmapped reads.

        For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

        Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Alignment metrics

        This module parses the output from samtools stats. All numbers in millions.

        loading..

        Samtools Flagstat

        This module parses the output from samtools flagstat. All numbers in millions.

        loading..

        XY counts

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Mapped reads per contig

        The samtools idxstats tool counts the number of mapped reads per chromosome / contig. Chromosomes with < 0.1% of the total aligned reads are omitted from this plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Picard (merged library; filtered)

        Picard (merged library; filtered) This section of the report shows picard results after merging libraries and after filtering.

        Alignment Summary

        Plase note that Picard's read counts are divided by two for paired-end data.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Base Distribution

        Plot shows the distribution of bases by cycle.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Mark Duplicates

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        deepTools

        deepTools This section of the report shows ChIP-seq QC plots generated by deepTools.

        Fingerprint plot

        Signal fingerprint according to plotFingerprint

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Read Distribution Profile after Annotation

        Accumulated view of the distribution of sequence reads related to the closest annotated gene. All annotated genes have been normalized to the same size. Green: -3.0Kb upstream of gene to TSS; Yellow: TSS to TES; Pink: TES to 3.0Kb downstream of gene

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        MACS2: Peak count

        MACS2: Peak count is calculated from total number of peaks called by MACS2

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        MACS2: Peak FRiP score

        MACS2: Peak FRiP score is generated by calculating the fraction of all mapped reads that fall into the MACS2 called peak regions. A read must overlap a peak by at least 20% to be counted. See FRiP score.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        spp: Strand-shift correlation plot

        spp: Strand-shift correlation plot generated using run_spp.R script from phantompeakqualtools.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        spp: NSC coefficient

        spp: NSC coefficient generated using run_spp.R script from phantompeakqualtools.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        spp: RSC coefficient

        spp: RSC coefficient generated using run_spp.R script from phantompeakqualtools.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        HOMER: Peak annotation

        HOMER: Peak annotation is generated by calculating the proportion of peaks assigned to genomic features by HOMER annotatePeaks.pl.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        nf-core/chipseq Software Versions

        nf-core/chipseq Software Versions are collected at run time from the software output.

        nf-core/chipseq
        v1.1.0
        Nextflow
        v19.10.0
        FastQC
        v0.11.8
        Trim Galore!
        v0.5.0
        BWA
        v0.7.17-r1188
        Samtools
        v1.9
        BEDTools
        v2.27.1
        BamTools
        v2.5.1
        deepTools
        v3.2.1
        Picard
        v2.19.0
        R
        v3.4.1
        Pysam
        v0.15.2
        MACS2
        v2.1.2
        featureCounts
        v1.6.4
        Preseq
        v2.0.3
        MultiQC
        v1.7

        nf-core/chipseq Workflow Summary

        nf-core/chipseq Workflow Summary - this information is collected when the pipeline is started.

        Run Name
        jolly_lumiere
        Data Type
        Single-End
        Design File
        design.csv
        Genome
        GRCh38
        Fasta File
        s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa
        GTF File
        s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf
        Gene BED File
        s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed
        BWA Index
        s3://ngi-igenomes/igenomes//Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa
        Blacklist BED
        /root/.nextflow/assets/nf-core/chipseq/assets/blacklists/hg38-blacklist.bed
        MACS2 Genome Size
        2.7e9
        Min Consensus Reps
        1
        MACS2 Narrow Peaks
        Yes
        Trim R1
        0 bp
        Trim R2
        0 bp
        Trim 3' R1
        0 bp
        Trim 3' R2
        0 bp
        NextSeq Trim
        0 bp
        Sequencing Center
        DFCI
        Fragment Size
        200 bp
        Fingerprint Bins
        500000
        Save Genome Index
        No
        Skip Diff Analysis
        Yes
        Skip Preseq
        Yes
        Max Resources
        128 GB memory, 24 cpus, 10d time per job
        Container
        docker - nfcore/chipseq:1.1.0
        Output Dir
        ./results
        Launch Dir
        /home/jeremie
        Working Dir
        /home/jeremie/work1
        Script Dir
        /root/.nextflow/assets/nf-core/chipseq
        User
        root
        Config Profile
        docker
        E-mail Address
        jkobject@gmail.com
        E-mail on failure
        N/A
        MultiQC Max Size
        25 MB